相关实验视频
Updated: Jul 15, 2025

08:43
OLIgo Mass Profiling OLIMP of Extracellular Polysaccharides
Published on: June 20, 2010
13.8K
从 Ziziphi Spinosae 精液中分离并阐明 prebiot oligosaccharides 的结构
Juan Song1, Yue Liu2, Xiangchang Yin1
1Department of Pharmaceutical Sciences, Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Carbohydrate research
|October 2, 2023
概括
六种来自Zhihihi Spinosae精液的新型寡糖体显示了益生菌效应,显著增加了有益的肠道细菌,如乳杆菌种类. 葡萄糖和拉芬糖是最有效的,突出了肠道健康的结构-活性关系.
科学领域:
- 自然产品化学 自然产品化学
- 微生物学 微生物学
- 营养科学 营养科学
背景情况:
- Ziziphi Spinosae精液是一种传统的草药,具有潜在的健康益处.
- 寡糖因其益生菌性质而闻名,影响肠道微生物群的组成.
- 了解新型寡糖的特定结构和 prebiotic 活动对于开发功能性食品和治疗药物至关重要.
研究的目的:
- 发现和描述从 Ziziphi Spinosae 精液中的新型寡糖类.
- 为了在体外评估这些寡糖类对肠道细菌生长的益生菌作用.
- 为了研究拉芬家族寡糖 (RFOs) 的结构-活性关系,关于它们对特定的乳酸菌种的影响.
主要方法:
- 用光谱分析从 Ziziphi Spinosae 精液中分离和阐明六种寡糖的结构.
- 在实验室测试了寡糖分量的前生物效应在八种不同的肠道细菌菌株.
- 评估单个寡糖的影响 (糖,拉芬等). 在 Lactobacillus acidophilus 的生长中具有不同程度的聚合 (DPs).
主要成果:
- 首次发现和分离了六种寡糖,包括白糖,白糖三糖,白糖三糖,白糖三糖,白糖三糖,白糖三糖,白糖三糖,白糖三糖和白糖三糖.
- 寡糖分数显著增加了Lactobacillus acidophilus,Lactobacillus gasseri和Lactobacillus johnsonii的细胞密度,增加了多达4倍.
- 与其他测试过的寡糖相比,葡萄糖和拉芬糖表现出更高的益生菌活性,更有效地支持Lactobacillus acidophilus的生长.
结论:
- Ziziphi Spinosae精液是具有显著前生物潜力的新型寡糖类的来源.
- 该研究确定了RFOs的结构-活性关系,表明具有更多样化的单糖类型和相似分子量的寡糖类更有利于有益的肠道细菌生长.
- 这些发现表明,Zhihihi Spinosae精液衍生的寡糖在调节肠道微生物群和促进肠道健康方面的潜在应用.
更多相关视频
11:49Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
Published on: March 24, 2016
7.7K
10:46Comprehensive Compositional Analysis of Plant Cell Walls Lignocellulosic biomass Part II: Carbohydrates
Published on: March 12, 2010
30.3K
相关概念视频
Oligosaccharide Assembly
2.9K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.9K
Biosynthesis of Polysaccharides
30
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
30