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Updated: Jul 15, 2025

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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在酵母和人体细胞中简化和敏感的单核和二核糖体概况

Lucas Ferguson1,2, Heather E Upton3, Sydney C Pimentel3

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA. lucas_ferguson@berkeley.edu.

Nature methods
|October 2, 2023
PubMed
概括

这项研究引入了一种使用P1核酶和简化协议的新核糖体分析方法. 这种方法减少了偏见和技术挑战,为研究翻译法规提供了更丰富的翻译组概况.

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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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