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Updated: Jul 15, 2025

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
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Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

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玉米半细胞和花粉粒的单细胞RNA-seq

Mary Washburn1, Jaime Alaniz-Fabián2, Taylor Scroggs1

  • 1University of Georgia, Athens, GA, USA.

Nature protocols
|October 2, 2023
PubMed
概括

这项研究提出了一种新方案,用于隔离单个玉米生殖细胞进行RNA测序 (RNA-seq). 这种方法可以对植物繁殖进行详细分析,有助于植物育种和遗传学研究.

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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis06:31

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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