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对两光子光两极性敏感探测器脂质滴的终身成像在体外和在体外

Jie Sha^1,2, Weimin Liu^1,2, Xiuli Zheng¹

¹Key Laboratory of Photochemical Conversion and Optoelectronic Materials and CityU-CAS Joint Laboratory of Functional Materials and Devices, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China.

Analytical chemistry

|October 3, 2023

概括

一个新的探测器可视化了脂质滴的极性变化,这种变化与诸如乳脂病等疾病有关. 这种工具可以进行深度成像,并为诊断与脂质相关的健康状况提供新的见解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
生物医学成像技术生物医学成像技术

背景情况:

脂质滴 (LD) 是脂质储存和新陈代谢的重要器官.
异常的LD聚合和极性转移与诸如牛排等疾病有关.
开发监测LD的工具对于理解和诊断这些疾病至关重要.

研究的目的:

设计和合成一种用于脂质滴滴成像的新型极度敏感探头.
为了研究探测器用于深层组织二光子成像的特性.
在疾病进展过程中可视化和分析LD的极性变化.

主要方法:

提供者-接受者-π-接受者 (D-A-π-A) 极性敏感探头 (TBPCPP) 的合成.
探测器光物理性质的表征,包括斯托克斯转移和两光子吸收 (TPA) 截面.
应用双光子光终身成像显微镜 (TP-FLIM) 用于细胞和体内成像.

主要成果:

合成的探头TBPCPP表现出大量的斯托克斯转移,高光稳定性和高效的LD准.
TBPCPP可进行深度两光子成像,可达~360μm,TPA截面为~226GM.
TP-FLIM成功地可视化了斑马鱼的HepG-2细胞和脂质丰富区域的LD极性变化,揭示了稳定症期间的极性下降.

结论:

TBPCPP是脂质滴滴成像的强大工具,对极性变化提供高灵敏度.
与TBPCPP一起的TP-FLIM提供了一种用于可视化LD积累和极性动态的新方法.
这种方法为诊断和了解乳脂病和其他LD相关疾病提供了新的见解.

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