通过 Bacillus subtilis Mz-12P 制造的药媒和点酶的产生
1Faculty of Pharmacy, Al-Sham Private University, Al-Tal Damascus, Syria.
Biotechnology and applied biochemistry
|October 3, 2023
概括
细菌Mz-12生产高产的稳定pectinase,一个酶对于工业应用至关重要. 这项研究优化了使用Pharmamedia的产量,这是这种酶的新方法.
科学领域:
- 微生物学 微生物学
- 酶学 是一种酶学.
- 生物技术是生物技术.
背景情况:
- pectinase酶对于各种工业过程至关重要,包括食品,织品和生物燃料生产.
- 选环境细菌寻找新型点酶生产者对于发现具有改进特性的酶至关重要.
- 细菌细菌是众所周知的工业相关酶的来源.
研究的目的:
- 从环境来源中分离和识别具有高解活性的细菌菌株.
- 为其潜在的工业应用而鉴定所选菌株产生的乳酶的特征.
- 为了优化来自Bacillus subtilis Mz-12的pectinase的产生和稳定性.
主要方法:
- 环境中的细菌分离物被选为乳酸分解活性.
- 最活跃的菌株Bacillus subtilis Mz-12被确定,其酸酶被净化和特征化.
- 对温度,pH值和生长介质进行了优化,在各种条件下评估了稳定性.
主要成果:
- 细菌素Mz-12显示出高的pectinase产量,在Pharmamedia中产生24.6U/mL,这是这种酶的新媒体.
- 纯化的构成性pectinase的分子量为22.3kDa.
- 该酶表现出广泛的稳定性,在pH9.0和50°C时具有最佳活性.
结论:
- 细菌亚菌Mz-12是工业规模生产pectinase的一个有前途的候选人.
- 描述的酸酶表现出有利的稳定性和活性,适用于工业用途.
- 这种菌株首次报告了Pharmamedia用于培菌酶的生产.
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