在C.中,凝聚素组织了围绕活性增强剂的3DDNA接触. 伊莱根斯 (elegans) 是一个词
Jun Kim1, Haoyu Wang1, Sevinç Ercan1
1Department of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA.
bioRxiv : the preprint server for biology
|October 3, 2023
概括
在没有CTCF的情况下,凝聚素组织了3D基因组,形成了DNA循环,在C. elegans中称为"喷泉". 这种保守的机制调节了动物基因组之间的增强剂相互作用.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 发展生物学 发展生物学
背景情况:
- 凝聚素和CTCF在哺乳动物中建立了3D基因组架构,调节了基因表达.
- 许多生物体,包括C. elegans,具有凝聚力,但缺乏CTCF,这表明了其他基因组组织机制.
研究的目的:
- 通过使用C. elegans. 在CTCF缺乏的生物体中研究凝聚素在3D基因组组织中的作用.
- 了解凝聚力介导的DNA循环的机制及其调节.
主要方法:
- 高通量染色体构造捕获 (Hi-C) 用于分析3D基因组结构.
- 染色体免疫沉测序 (ChIP-seq) 用于绘制蛋白质结合部位的地图.
- 凝聚素和WAPL-1的耗尽研究,以评估它们的功能影响.
主要成果:
- 在C. elegans中确定了"喷泉",凝聚素依赖的DNA循环 (~20-40 kb),跨物种保持.
- 凝聚性加载优先发生在NIPBL站点,并双向挤出循环.
- 凝聚蛋白转位取决于完整的复合体,并由WAPL-1调节,观察到不平等的过程性.
结论:
- 凝聚素介导循环挤出是调节3D基因组组织和增强剂相互作用的进化保守机制.
- 与哺乳动物相比,C. elegans的凝聚力表现出较短的过程性和明显的NIPBL结合.
- 在活性增强剂中偏好的凝聚蛋白负载与转录调节有关.
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