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杂交导致了复合性多能性网络的重新连接在allotetraploid Xenopus laevis 中

Wesley A Phelps¹, Matthew D Hurton¹, Taylor N Ayers¹

¹Department of Biological Sciences, University of Pittsburgh, Pittsburgh, United States.

|October 3, 2023

概括

在Xenopus laevis中,OCT4和SOX2因子不对称地激活同源物,尽管基因组不稳定性来自allotetraploidy. 由于选择压力,基因剂量在早期发育过程中保持不变.

科学领域:

发展生物学发展生物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

母亲因素在受精后启动多能性和胚胎干细胞的形成.
这种过渡涉及激活胚胎基因组转录,在物种之间有不同的机制.
来自杂交的Xenopus laevis的Allotetraploidy为研究早期发育提供了一个独特的模型.

研究的目的:

研究OCT4和SOX2同类物如何在Xenopus laevis中差异激活两个亚基因组.
了解全四化对基因调节和增强器架构的影响.
在基因组不稳定的背景下,研究胚胎基因表达和剂量的保存.

主要方法:

染色体可访问性概况.
用CUT&RUN检测修饰的基因素和转录因子结合.
与双胞胎Xenopus tropicalis和斑马鱼进行比较分析.

主要成果:

母亲的OCT4和SOX2同类物不同激活Xenopus laevis的两个亚基因组.
大多数保留的同源基因在早期胚胎中显示不对称的激活.
亚基因组之间的增强器架构的差异与来自allotetraploidy的基因组破坏有关.

结论:

在Xenopus laevis的杂交后的基因组不稳定导致不对称的基因激活.
尽管有不同的同源贡献,但由于强烈的选择,核心脊椎动物多能性基因剂量得到保留.
这突显了基因组进化和发育调节之间的相互作用.

关键词:

所有多重复合体性.进行比较的基因组学.发育生物学是发展生物学.进化生物学是进化的生物学.基因组的演化基因组的演化多能性的多能性.异国主义者 (Xenopus) 是紫芽基因组激活活动

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