小殖民因子CS14在地理上多样化的ETEC分离物对肠道细胞模型的坚持中的作用
Emily M Smith1, Antonia Papadimas1, Caitlin Gabor1
1Center for Vaccine Development and Global Health, University of Maryland School of Medicine , Baltimore, Maryland, USA.
mSphere
|October 3, 2023
概括
肠毒性大肠杆菌 (ETEC) 会导致严重的腹. 这项研究表明,CS14是一种轻微的殖民因素,对ETEC的坚持和潜在的疫苗目标至关重要.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 肠毒性大肠杆菌 (ETEC) 是低收入至中等收入国家的旅行者和儿童腹的主要原因.
- ETEC使用殖民因子 (CF) 粘附于小肠,导致水性腹.
- CS14是一种轻微的CF,被确定与中度至重度腹 (MSD) 有显著的关联,并推用于ETEC疫苗.
研究的目的:
- 研究ETEC中CS14殖民因子的序列保护,粘附作用和交叉反应.
- 评估CS14作为ETEC疫苗的潜在抗原.
主要方法:
- 在各种ETEC分离物中对CS14操作子的序列分析.
- 西方斑点和电子显微镜用于评估CS14表达.
- 使用培养肠道细胞和人类肠体进行粘附测试.
- 使用抗CS14,抗CS4和抗CFA/I抗体进行抑制试验.
主要成果:
- CS14操作子在分离物中显示了>99.9%的序列一致性,与CsuD粘附基因中的SNP相匹配.
- 铁化诱导了CS14的表达,并显著增加了细菌对肠道细胞和肠杆菌的附着性.
- 反-CS14和反-CS4抗体抑制了附着性,表明CS14特异性抑制和5a类fimbriae中的部分交叉反应性.
结论:
- CS14是ETEC的保存和重要的手指细胞殖民因子.
- CS14在ETEC粘附于肠上皮质方面发挥着重要作用.
- CS14是一种有希望的候选抗原,可用于未来的ETEC疫苗中.
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