通过T细胞受体测序激活的CD8+T细胞来诊断病毒感染
Alexandra Vujkovic1,2,3, My Ha2,4,5,6, Tessa de Block7
1Clinical Virology Unit, Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
The Journal of infectious diseases
|October 3, 2023
概括
这项研究引入了T细胞受体 (TCR) RNA测序,以区分最近的2019年冠状病毒病 (COVID-19) 感染与过去的严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 暴露. 这种基于TCR的新型诊断方法准确地区分了急性病毒感染.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 生物信息学是一种生物信息学.
背景情况:
- T细胞诊断可以检测病原体暴露,但很难区分最近和历史感染.
- 从临床上来说,区分2019年急性冠状病毒病 (COVID-19) 与之前的严重急性呼吸综合征冠状病毒2 (SARS-CoV-2) 暴露是非常重要的.
研究的目的:
- 开发和验证一种T细胞受体 (TCR) RNA测序方法,用于诊断急性病毒感染.
- 使用TCR剧目分析,区分最近的COVID-19和历史的SARS-CoV-2暴露.
主要方法:
- 在住院COVID-19患者和健康对照者的CD8+T细胞子集上进行了T细胞受体 (TCR) RNA测序.
- 对CDR3α和CDR3βTCR序列进行了集群,并对超过1000个SARS-CoV-2表位元的数据库进行了表位元特异性的注释.
- 分析了与SARS-CoV-2相关的TCR序列的深度,以区分患者组.
主要成果:
- 对TCR序列的分析成功地将COVID-19患者与健康对照者区分开来,包括那些先前接触过SARS-CoV-2的人.
- 在0.84±0.10的曲线下实现了接收器运行特征面积,证明了高诊断精度.
- TCR序列的深度和特异性与急性病毒感染状态相关.
结论:
- 标注激活CD8+T细胞的TCR序列为诊断COVID-19等急性病毒感染提供了一种新的方法.
- 这种基于TCR的诊断策略可以有效地区分急性感染和以前接触过的病原体,如SARS-CoV-2.
- 这项研究为诊断中的TCR谱系应用建立了新的范式.
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