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基于金属有机框架的杀虫剂和dsRNA代码交付系统用于管理抗虫剂耐药性

Chang Yu¹, Jiaqing Li¹, Zhaoyang Zhang¹

¹The Center of Crop Nanobiotechnology, College of Plant Science and Technology, Huazhong Agricultural University, No. 1 Shizishan Street, Wuhan 430074, China.

ACS applied materials & interfaces

|October 3, 2023

概括

一个新的纳米粒子系统有效地提供杀虫剂和双链RNA (dsRNA) 来向昆虫害虫. 这种双重作用的方法显著提高了杀虫剂的有效性,为管理作物的抗虫剂耐药性提供了一个有希望的策略.

科学领域:

农业昆虫学农业昆虫学
纳米技术在害虫管理中的作用
分子昆虫学分子昆虫学

背景情况:

像Nilaparvata lugens (Stål) 这样的害虫对杀虫剂的耐药性对作物保护构成了重大挑战.
使用双链RNA (dsRNA) 的基因沉默是克服抗虫剂耐药性的潜在策略.
有效地将dsrna和杀虫剂输送到目标害虫仍然是一个障碍.

研究的目的:

开发一种用于杀虫剂 (imidacloprid) 和针对耐药性相关基因NlCYP6ER1.1的dsRNA的代码传递系统.
通过基于纳米粒子的方法,提高尼拉帕尔瓦塔 (Nilaparvata lugens) 对伊米达克洛普里德的敏感性.
评估代码交付系统的有效性和生物安全性.

主要方法:

封装的伊米达克洛普里德和dsNlCYP6ER1 在化伊米达酸框架-8 (ZIF-8) 纳米颗粒.
伊米达克洛普里德/dsNlCYP6ER1@ZIF-8纳米粒子的表征 (大小,电荷,单分散性).
使用Nilaparvata lugens进行生物测试,以评估治疗后的生存率.
测试实验,以评估现场有效性.
基因表达分析和光追踪用于交付确认.
对丝虫的生物安全评估.

主要成果:

这种imidacloprid/dsNlCYP6ER1@ZIF-8纳米粒子 (195纳米,正电荷) 有效地配送了dsRNA和杀虫剂.
与伊米达克洛普里德/dsNlCYP6ER1@ZIF-8 治疗显著降低了尼拉帕尔瓦塔 (Nilaparvata lugens) 的存活率 (8%的存活率),与单独使用伊米达克洛普里德 (82%的存活率) 相比.
子实验显示,使用代码交付系统的生存率从54%大幅下降到24%.
通过米植物,ZIF-8纳米颗粒成功地传递了dsRNA和杀虫剂.
dsNlCYP6ER1@ZIF-8纳米粒子表现出良好的生物相容性和生物安全性.

结论:

开发的基于ZIF-8的代码传递系统通过沉默Nilaparvata lugens的耐药基因,有效地提高了杀虫剂的有效性.
这种纳米粒子系统为克服抗虫剂耐药性和改善害虫管理提供了可行的策略.
代码交付方法有可能与其他面临耐药性问题的杀虫剂一起应用.

关键词:

尼拉帕尔瓦塔·卢根斯 (Stål) 公司一个NlCYP6ER1一个.这里是ZIF-8的位置.代码交付是指提供代码.伊米达克洛普里德 (Imidacloprid) 是一种

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