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三功能链接器通过扩展显微镜使动素的可视化得到改善

Gang Wen^1,2, Matthew Domenic Lycas², Yuqing Jia³

¹Department of Chemistry, KU Leuven, Leuven, 3001, Belgium.

|October 3, 2023

概括

优化的三功能链接器 (TRITON) 显著增强使用扩展显微镜 (ExM) 的活性丝可视化. 这种方法使信号保留率提高了10倍,使细胞和组织中actin结构的详细成像成为可能.

科学领域:

生物技术是生物技术.
显微镜的使用方法
细胞生物学细胞生物学

背景情况:

扩展显微镜 (ExM) 能够在标准显微镜上进行超高分辨率成像.
使用ExM有效地可视化actin纤维仍然是一个挑战.
以前试图用多功能分子改进actin成像的尝试取得了中等的成功.

研究的目的:

开发针对法洛伊丁结合物移植的优化方法,用于ExM中增强的actin成像.
引入和评估三功能链接器 (TRITON) 以改善动态可视化.
在各种ExM协议和样本类型中证明TRITON链接器的有效性.

主要方法:

优化了法洛伊丁合物移植策略.
三功能连接器 (TRITON) 的开发和应用.
使用ExM和Airyscan共聚焦显微镜在培养的神经元和大脑切片中的actin结构的成像.

主要成果:

优化的接种策略大约提高了10倍的定和信号保留.
在细胞和组织样本中,TRITON链接器在actin成像方面表现出高效率.
10X ExM与TRITON标记的actin允许可视化神经元和大脑切片中的actin环周期性.

结论:

在ExM中,TRITON链接器提供了一种有效的Actin可视化方法.
当目标结合的单体度较低时,这种方法特别有益.
使用TRITON优化ExM可增强对生物样本中富含actin的结构的研究.

关键词:

亚丁丝是一种亚丁丝.扩张显微镜扩张显微镜多色成像技术的多色成像技术神经元神经元的神经元超级分辨率的超级分辨率三功能的法洛伊丁链接器

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