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在耐吉他的膀癌中构建和识别lncRNA/circRNA-coregulated ceRNA网络

Jingjing Pan¹, Xiaojuan Xie², Jinxiu Sheng¹

¹Department of Clinical Laboratory, The First Affiliated Hospital of Zhengzhou University and the Key Clinical Laboratory of Henan Province, Zhengzhou, China.

|October 3, 2023

概括

研究人员发现了涉及长非编码RNAs (lncRNAs) 和循环RNAs (circRNAs) 的调节网络,这些网络在膀癌中驱动化学抵抗. 这些发现揭示了对gemcitabine耐药性的关键机制和潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

抗化学剂是膀癌治疗的一个主要挑战.
了解化学抵抗的分子机制对于开发有效疗法至关重要.

研究的目的:

探索膀癌中基因化学抵抗的调节网络.
为了确定关键的长非编码RNAs (lncRNAs),圆形RNAs (circRNAs),微RNAs (miRNAs) 和信使RNAs (mRNAs) 参与凝素耐药性.

主要方法:

在使用下一代测序的gemcitabine耐药/敏感膀癌细胞中对lncRNAs,cirRNAs,miRNAs和mRNAs的差异表达分析.
生物信息学分析包括染色体定位,分类,协同表达和 cis/trans 调节.
基因本体学和基因和基因组通路丰富分析的京都百科全书.
竞争性内源RNA (ceRNA) 和蛋白质与蛋白质相互作用网络的构建.

主要成果:

数以百计的差异表达的lncRNAs和miRNAs,以及成千上万的circRNAs和mRNAs被确定.
生物信息学分析揭示了复杂的调节网络和共同调节的RNA的功能作用.
具体例子包括 lncRNA LINP1 作为 miR-193a-5p 的 ceRNA 来增加 TP73 的表达,以及 lncRNA ESRG 和 hsa_circ_0075881 结合 miR-324-3p 来增加 ST6GAL1 的表达.
对ceRNA网络组件的调制影响了膀癌细胞中的凝胺耐药性.

结论:

在膀癌中lncRNA和circRNA协同调节的阐明机制.
建立了识别新生物标志物和膀癌化学抵抗治疗标的基础.

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