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粉样β表层和异构体的可变碎片化和电离

Elizabeth R Readel¹, Umang Dhaubhadel¹, Arzoo Patel¹

¹Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, 76019, USA.

Analytical and bioanalytical chemistry

|October 3, 2023

概括

阿尔茨海默病中的D-氨基酸由于质量相似,难以分析. 新的质谱法揭示了碎片化差异,有助于研究这些重要的变异.

科学领域:

生物化学生物化学
分析化学分析化学
神经科学是一个神经科学.

背景情况:

和蛋白质中的D-氨基酸在更高的生物体中越来越多地被识别出来,但它们的功能仍然不清楚.
经常观察到表皮和异构修饰,特别是在阿尔茨海默病患者的粉样ββ (Aβ) 中.
由于质量相同,现有的分析方法难以区分这些改性.

研究的目的:

研究含有D-Asp,L-iso-Asp,D-iso-Asp和D-Ser修饰的三性Aβ的MS/MS碎片化模式.
探索这些修改对的电离性和碎片化的影响.
突出选择性分析技术的必要性,以区分异构和表构.

主要方法:

对三重性Aβ片段的MS/MS碎片化的分析.
利用选择反应监测 (SRM),选择离子监测 (SIM) 和产品离子扫描技术.
检查了具有D-Asp,L-iso-Asp,D-iso-Asp和D-Ser修饰的.

主要成果:

对于具有相同质量但不同立体化学的酸,观察到电离和碎片化的显著变异.
这些变异证明了使用MS/MS.区分表皮和异皮的潜力.
该研究确定了可以用于选择性分析的特定碎片化差异.

结论:

质谱法/质谱法 (MS/MS) 可以区分异构和表构Aβ.
了解碎片变异性对于准确分析阿尔茨海默氏症等疾病中修饰的准确分析至关重要.
先进的分析方法对于研究D-氨基酸在生物系统中的作用至关重要.

关键词:

阿尔茨海默氏症是阿尔茨海默氏症的一种疾病.粉样蛋白β是什么粉样蛋白β是什么它们是表皮层.碎片化碎片化电离化的电离.质谱测量质量谱测量

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