Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jul 15, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.0K

集成的微波阵列基于微流体芯片与手持智能手机控制的设备用于核酸检测.

Haiying Shen1, Lianhua Dong1, Yunhua Gao1

  • 1National Institute of Metrology, Beijing 100029, People's Republic of China.

Analytical chemistry
|October 3, 2023
PubMed
概括

本研究介绍了一种集成的微流体芯片,用于快速核酸检测. 这种便携式,智能手机控制的设备可以在任何有互联网接入的地方轻松进行临床检测.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Highly Integrated Microfluidic Analyzer for Both Chemiluminescence Immunoassay and Fluorescence LAMP Detection with an Enhanced Cooled CMOS Camera.

Analytical chemistry·2026
Same author

Benchmarking Q40 sequencing for sensitive and efficient detection of rare genomic variants.

Genome biology·2026
Same author

Pseudovirus reference material and RT-digital PCR reference method for the quantification of human rhinovirus RNA with metrological traceability.

Clinical chemistry and laboratory medicine·2026
Same author

A metrological foundation for absolute transcriptomics using International System of Units-anchored calibrators.

Nature communications·2026
Same author

A Telomere-to-telomere Diploid Reference Genome and Centromere Structure of the Chinese Quartet.

Genomics, proteomics & bioinformatics·2025
Same author

Methylation reference datasets from quartet DNA materials for benchmarking epigenome sequencing.

Nature communications·2025

科学领域:

  • 生物技术是生物技术.
  • 微流体学 微流体学
  • 分子诊断学 分子诊断学

背景情况:

  • 核酸检测对于疾病诊断至关重要.
  • 目前的方法可能很复杂,需要专门的设备.
  • 需要便携式,用户友好的诊断平台.

研究的目的:

  • 设计一个高度集成的微流体芯片用于核酸提取,放大和检测.
  • 开发一个紧的,手持设备来操作芯片.
  • 为了使智能手机控制,互联网支持的核酸测试.

主要方法:

  • 在微波阵列中利用磁珠捕获核酸.
  • 通过手动注射器操作开发了芯片上的液体处理.
  • 集成了一个手持设备,具有温度控制和成像功能.
  • 采用反转录循环介导的同热放大 (RT-LAMP).

主要成果:

  • 实现了"一个井一个珠"的磁珠捕获.
  • 证明了成功的核酸提取,放大和检测SARS-CoV-2伪病毒.
  • 验证了手持设备对RT-LAMP的性能.
  • 通过互联网连接确认了智能手机控制和数据分析.

结论:

  • 成功开发了一种高度集成,易于操作的微流体芯片和手持设备.
  • 该平台可以快速检测核酸.
  • 该系统显示出移动点诊测试 (POCT) 的巨大潜力.

更多相关视频

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.0K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.2K

相关实验视频

Last Updated: Jul 15, 2025

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

15.0K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

15.0K
A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

17.2K