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集成的微波阵列基于微流体芯片与手持智能手机控制的设备用于核酸检测
Haiying Shen1, Lianhua Dong1, Yunhua Gao1
1National Institute of Metrology, Beijing 100029, People's Republic of China.
Analytical chemistry
|October 3, 2023
概括
本研究介绍了一种集成的微流体芯片,用于快速核酸检测. 这种便携式,智能手机控制的设备可以在任何有互联网接入的地方轻松进行临床检测.
科学领域:
- 生物技术是生物技术.
- 微流体学 微流体学
- 分子诊断学 分子诊断学
背景情况:
- 核酸检测对于疾病诊断至关重要.
- 目前的方法可能很复杂,需要专门的设备.
- 需要便携式,用户友好的诊断平台.
研究的目的:
- 设计一个高度集成的微流体芯片用于核酸提取,放大和检测.
- 开发一个紧的,手持设备来操作芯片.
- 为了使智能手机控制,互联网支持的核酸测试.
主要方法:
- 在微波阵列中利用磁珠捕获核酸.
- 通过手动注射器操作开发了芯片上的液体处理.
- 集成了一个手持设备,具有温度控制和成像功能.
- 采用反转录循环介导的同热放大 (RT-LAMP).
主要成果:
- 实现了"一个井一个珠"的磁珠捕获.
- 证明了成功的核酸提取,放大和检测SARS-CoV-2伪病毒.
- 验证了手持设备对RT-LAMP的性能.
- 通过互联网连接确认了智能手机控制和数据分析.
结论:
- 成功开发了一种高度集成,易于操作的微流体芯片和手持设备.
- 该平台可以快速检测核酸.
- 该系统显示出移动点诊测试 (POCT) 的巨大潜力.
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