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循环RNA对胚胎低氧适应的调节作用

Xuejiao Chen¹, Ying Zhang², Wenhui Zhang¹

¹State Key Laboratory of Farm Animal Biotech Breeding, Beijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

Journal of animal science

|October 3, 2023

概括

西藏具有独特的适应能力,能够适应高海拔地区的低氧. 这项研究确定了调节基因表达的特定循环RNA (circRNAs),这可能解释了它们在低氧环境中的生存机制.

科学领域:

基因组学就是基因组学.
分子生物学分子生物学
动物适应动物适应

背景情况:

西藏原产于高海拔高原,具有独特的适应缺氧条件.
循环RNAs (circRNAs) 是参与调节基因表达和血管生成的内源分子,对缺氧适应至关重要.

研究的目的:

鉴定和描述西藏中的circRNA及其在高海拔低氧适应中的作用.
为了研究藏人中低氧耐受性背后的分子机制.

主要方法:

Ribo-Zero RNA测序在藏族和查华胚胎化在低氧环境中的胆管质膜上进行.
进行了circRNAs,microRNAs (miRNAs) 和信使RNAs (mRNAs) 的差异表达分析.
用竞争性内源RNA (ceRNA) 网络分析来确定调节途径.

主要成果:

总共有1,414个circRNAs被确定,其中93个在西藏和查华之间有差异表达.
四个特定的circRNAs (circBRD1,circPRDM2,circPTPRS,circDENND4C) 被确定为关键的调节者.
这些circRNAs可以作为ceRNAs来通过 novel_miR_589调节APOA1表达,从而影响血管生成和缺氧适应.

结论:

已识别的circRNAs, novel_miR_589和APOA1形成了一个调节轴,该轴对西藏的缺氧适应至关重要.
这种circRNA介导的调节网络提供了对平原低氧适应的分子机制的洞察.
这些发现为了解 circRNAs 在鸟类适应极端环境中的功能性作用提供了有价值的证据.

关键词:

西藏的肉,西藏的肉.圆形的RNARNA是一个圆形的RNA.低氧适应适应低氧适应监管机制监管机制

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