甲基炭酸盐会破坏Streptococcus suis的生物膜结构,并对抗囊中的多糖体防御效应
Shuji Gao1, Yamin Shen1, Shuo Yuan1
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China; Henan Provincial Engineering Research Centre for Detection and Prevention and Control of Emerging Infectious Diseases in Livestock and Poultry, Luoyang, China.
International journal of antimicrobial agents
|October 3, 2023
概括
甲基炭酸盐 (MA) 通过抑制囊多糖化合物合成和定数感应来破坏Streptococcus suis生物膜. 这种化合物显示出作为一种新型治疗剂对抗S. suis感染的潜力.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 药物发现 药物发现 药物发现
背景情况:
- 瑞士杆菌 (Streptococcus suis) 是一个重要的动物传染病原体.
- 在*S. suis*感染中形成生物膜会导致药物耐药性增加.
- 对*S. suis*生物膜感染需要新的治疗方法.
研究的目的:
- 为了研究甲基乙烯酸盐 (MA) 对*S. suis*生物膜的影响.
- 阐明MA对*S. suis*采取行动的机制.
- 评估MA作为潜在的治疗剂.
主要方法:
- 水晶紫色测定和电子显微镜用于生物膜结构分析.
- 醇 - 硫酸方法和HPLC用于囊聚糖的量化.
- 细胞对粘附和抗细胞酶的测定.
- 分子对接,模拟和酶抑制试验用于定数感应 (QS) 分析.
- 用于治疗评估的老鼠感染模型.
主要成果:
- MA显著破坏了现有的生物膜,并抑制了新的生物膜形成.
- 亚马降低了囊多糖合成,削弱了细菌的粘附和抗细胞分裂.
- 通过与5'-甲基氨酸/S-氨酸同型氨酸核糖酶结合,MA竞争性地抑制了AI-2 QS.
- 在小鼠模型中,MA治疗有效降低了细菌负载和炎症.
结论:
- 甲基炭酸盐表现出强大的抗菌膜特性,可以对抗*Streptococcus suis*.
- 麻醉剂作为检定人数感应抑制剂,提供了一个新的治疗策略.
- 马氏菌对治疗*S. suis*感染具有显著的潜力.
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