茶,儿茶素和儿茶素衍生物对SARS-CoV-2的Omicron亚变体的影响
Masaharu Shin-Ya1,2, Maiko Nakashio1,3, Eriko Ohgitani1
1Department of Immunology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Scientific reports
|October 3, 2023
概括
像EGCG和TFDG这样的绿茶化合物通过向S蛋白受体结合域,有效地使Omicron亚变体无活化. 饮用茶减少了唾液中的病毒感染力,这表明未来流行病病毒抑制的潜力.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 由于S-蛋白的突变,SARS-CoV-2的Omicron亚型具有很高的传染性.
- (-) -epigallocatechin gallate (EGCG) 和theaflavin-3,3'-di-O-digallate (TFDG) 通过与S蛋白的受体结合域 (RBD) 结合,先前已经使SARS-CoV-2失活.
研究的目的:
- 调查EGCG和TFDG对Omicron亚型的疗效.
- 探索这些化合物与病毒RBDs之间的相互作用背后的分子机制.
- 评估茶叶消费在减少病毒感染性方面的潜力.
主要方法:
- 在使用Omicron亚变体 (BA.1,XE,BA.2.75) 的体外无活化试验.
- 中和测试用于评估RBD-ACE2相互作用的抑制.
- 在基分析中预测结合相互作用并识别关键突变.
- 人类志愿者研究涉及茶饮和唾液分析.
主要成果:
- 绿茶,抹茶和黑茶有效地禁用了Omicron亚型.
- EGCG和TFDG显示出强烈抑制BA.1和XE感染性,对BA.2.75.5的影响较弱.
- 中和测定证实EGCG和TFDG抑制BA.1 RBD-ACE2相互作用.
- 在分析中发现了影响化合物结合的特定RBD突变 (N460K,G446S,F490S).
- 摄入茶叶的志愿者的唾液在体外显著降低了BA.1的感染性.
结论:
- 在Omicron RBD中,特定的氨基酸替代物会影响EGCG/TFDG结合,从而导致不同的亚变体易感性.
- 这些茶叶化合物显示出抑制新兴突变SARS-CoV-2菌株的潜力.
- 茶饮可能提供一种随时可用的方法来减少病毒感染.
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