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Updated: Jul 15, 2025

Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
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对于Enterobacteriaceae的阿拉比诺酸诱导人工转录因子的一套多功能调控工具

Gita Naseri1,2, Hannah Raasch3, Emmanuelle Charpentier4,3

  • 1Max Planck Unit for the Science of Pathogens, Charitéplatz 1, 10117, Berlin, Germany. naseri@mpusp.mpg.de.

Communications biology
|October 3, 2023
PubMed
概括

研究人员开发了新的人工转录因子 (ATF),用于控制大肠杆菌和沙门氏菌的基因表达. 这些ATF能够为生物传感器开发和增强生物生产提供精确的调节,从而推进合成生物学应用.

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The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
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Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
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