循环酶DEP1:其在叶子衰老期间在PSI和ROS生产中的月光功能
Chu-Kun Wang1, Xiu-Ming Li1, Fang Dong2
1National Key Laboratory of Crop Biology; MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, Shandong, China.
果脱水酶-酸酶-酸酶-复合物1 (MdDEP1) 破坏MdY3IP1的稳定,减少光系统I的活动,并将叶子衰老与光合作用衰退同步.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 乙烯信号对于叶子衰老和光合作用调节至关重要.
- 连接乙烯,光合作用和衰老的精确分子机制在很大程度上是未知的.
研究的目的:
- 研究果DEHYDRATASE-ENOLASE-PHOSPHATASE-COMPLEX1 (MdDEP1) 在调节叶子衰老和光合作用中的作用.
- 在乙烯信号传输的背景下,阐明MdDEP1及其目标之间的分子相互作用.
主要方法:
- 酵母二杂交 (Y2H) 测定方法
- 拉下测试试剂的测试方法
- 同免疫沉 (CO-IP) 是一种共免疫沉.
- 无细胞降解试验试验.
- 生物化学测定 生物化学测定
主要成果:
- MdDEP1直接与核编码的甲状腺蛋白蛋白MdY3IP1相互作用并脱.
- 通过MdDEP1介导的MdY3IP1脱化导致其不稳定.
- 这一过程导致光系统I (PSI) 活性降低,反应性氧物种 (ROS) 生产增加.
- MdDEP1 负面调节了叶子衰老.
结论:
- MdDEP1具有月光作用,将乙烯信号与光系统I活动和ROS平衡联系起来.
- MdDEP1 破坏了 MdY3IP1 的稳定,从而同步了以乙烯为媒介的叶子衰老与光合作用的妥协.
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