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人类肠道细菌C-Glycoside-Metabolizing人类肠道细菌,多雷亚sp. 在MRG-IFC3中,

Huynh Thi Ngoc Mi¹, Santipap Chaiyasarn¹, Heji Kim¹

¹Metalloenzyme Research Group and Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.

Journal of microbiology and biotechnology

|October 4, 2023

概括

研究人员分离了一种新的肠道细菌,Dorea sp. MRG-IFC3,能够在像 puerarin 这样的异黄 C-糖化物中切割 C-C 糖化物键. 这一发现促进了对肠道微生物代谢和生物活性化合物转化的理解.

科学领域:

微生物学微生物学
生物化学生物化学
肠道微生物组研究研究

背景情况:

饮食中的生物活性化合物经历肠道代谢,在分子水平上显著影响健康.
了解这些转变的生物化学途径对于阐明与健康有关的问题至关重要.
肠道细菌对稳定的C-糖酸键的裂变仍然是研究的一个关键领域.

研究的目的:

选和分离人类肠道细菌,能够裂解C-C甘氨酸键.
对于C-糖酸和O-糖酸的分离细菌的基质特异性进行表征.
通过同位素标记和光谱分析,研究C-糖酸键裂解的机制.

主要方法:

在无氧条件下对人类便样本进行查,以检测降解C-糖化的细菌.
隔离和鉴定了一种新的阳性菌株,Dorea sp. 在MRG-IFC3.3.
用各种C-糖和O-糖进行生物转化测定,然后在D2O GAM介质中进行1H NMR光谱,以阐明裂变机制.

主要成果:

一种新的C-糖酸脱糖化菌株,Dorea sp. 隔离了MRG-IFC3,它能够将氨酸转化为大氨酸.
MRG-IFC3表现出特异性,可以代谢异黄C-糖化物和一些7-O-糖化物,但不能代谢其他C-糖化物或黄3-O-糖化物.
一小时的NMR证实了D2O中的puerarin产生的daidzein在C-8位置上的丰富,支持了特定的裂变机制.

结论:

多雷亚种类多雷亚种类 Dorea sp. MRG-IFC3是一种新型肠道细菌,具有分裂特定C-C甘酸键的能力,特别是在异黄中.
这项研究提供了第一个直接证据,证明了肠道细菌通过C-糖键裂解从皮亚林中产生大idzein.
拟议的反应中间体为了解肠道中稳定的C-糖酸键水解的机制提供了分子基础.

关键词:

C-C 债券裂变的发生.在C-glycoside的使用中.多雷亚种类多雷亚种类 Dorea sp. 在MRG-IFC3中,肠道代谢肠道代谢波尔多斯人是什么意思

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