雌性在交配期间转移的雄性mRNA进行翻译
Luciano M Matzkin1,2,3, Jeremy M Bono4, Helen K Pigage4
1Department of Entomology, University of Arizona, Tucson, AZ, USA.
bioRxiv : the preprint server for biology
|October 4, 2023
概括
雄性精液RNA被雌性果翻译成67种新的蛋白质,这些蛋白质影响生殖和性冲突. 这一发现揭示了两性之间的新型分子相互作用.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 在许多物种的精液中都存在RNA.
- 在女性体内,精液RNA的功能在很大程度上是未知的.
- 了解交配后的分子相互作用对于生殖生物学至关重要.
研究的目的:
- 调查男性精液RNA是否具有功能性,并由 *Drosophila* 的雌性进行翻译.
- 为了识别由雌性在交配后翻译的男性衍生的蛋白质.
- 探索这些蛋白质在交配后生殖相互作用中的作用.
主要方法:
- 开发一种新型蛋白质组学方法,变种启用SILAC蛋白质组学分析 (VESPA).
- 对女性生殖道的定量蛋白质组分析.
- 基因淘汰实验用于评估已识别的蛋白质的功能.
主要成果:
- 在女性生殖系统中鉴定67种男性衍生,女性转化蛋白 (mdFTPs).
- 许多已识别的mdFTP具有与繁殖相关的功能.
- 基因淘汰揭示了mdFTPs影响受精效率和受精反应的质量形成/持久性.
结论:
- 男性的精液RNA可以被女性翻译,这代表了一种新的交配后分子相互作用机制.
- 这些发现揭示了以前未知的男性影响生殖结果的方法.
- 这一发现对了解跨物种繁殖中的合作和冲突有着广泛的影响.
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