一种基于呼吸的体外诊断,用于下呼吸道感染
Dapeng Chen1, Marek A Mirski2, Emily R Caton1
1Zeteo Tech, Inc., Sykesville, MD 21784, United States.
medRxiv : the preprint server for health sciences
|October 4, 2023
概括
一种新的呼吸测试通过测量蛋白酶活性来检测下呼吸道感染 (LRTI). 这种非侵入性方法使用分子传感器和质谱仪进行LRTIs的特定和早期诊断.
科学领域:
- 生物化学 生物化学
- 医学诊断 医学诊断 医学诊断
- 分析化学 分析化学
背景情况:
- 下呼吸道感染 (LRTI) 是传染病死亡的主要原因.
- 目前对LRTIs的诊断方法缺乏特异性,经常将殖民与感染混为一谈.
- 对LRTIs而言,非常需要非侵入性和准确的诊断工具.
研究的目的:
- 为LRTIs开发一种非侵入性诊断方法.
- 在人类呼吸中检测细菌感染诱导的蛋白酶活动.
- 为诊断目的设计一种针对人类中性粒细胞弹性酶 (HNE) 的特定传感器.
主要方法:
- 使用了BreathBiomicsTM,一个人类呼吸采样系统.
- 为HNE设计了一种高灵敏度的分子传感器.
- 通过矩阵辅助激光吸附/离子化飞行质谱时间 (MALDI-TOF MS) 检测到传感器裂变.
主要成果:
- 成功检测到裂开传感器的呼吸样本从直管患者LRTIs.
- 证明了 MALDI-TOF MS 识别裂开传感器的能力.
- 验证了HNE传感器的特异性.
结论:
- 开发的呼吸分析方法为LRTIs提供了一个非侵入性的诊断策略.
- 与传统的临床基于症状的诊断相比,这种方法提供了更好的特异性.
- 这项技术有望更早,更准确地检测下呼吸道感染.
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