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在巨细胞中通过FABP5调节的信号通路的蛋白质组分析
Faniya Doswell1,2, John D Haley3,4, Martin Kaczocha2
1Molecular and Cellular Biology Program, Stony Brook University, Stony Brook, NY, USA.
Research square
|October 4, 2023
概括
脂肪酸结合蛋白5 (FABP5) 在巨细胞中被删除会改变炎症和激酶通路. 这项蛋白质组学研究揭示了FABP5在巨细胞极化中的作用,并表明在炎症性疾病中抑制FABP5的治疗潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 慢性炎症与许多疾病有关,并涉及巨细胞两极分化.
- 脂肪酸结合蛋白5 (FABP5) 在巨细胞中高度表达,并影响其炎症表型.
- 巨细胞FABP5调节的信号通路需要系统的分析.
研究的目的:
- 为了全面描述M1和M2极化骨髓衍生巨细胞 (BMDMs) 具有和没有FABP5.5的蛋白质和蛋白质景观.
- 在极化巨细胞中识别由FABP5调节的关键信号通路和激酶.
主要方法:
- 使用氨基酸稳定同位素标记 (SILAC) 进行定量蛋白质和蛋白质分析.
- 在M1和M2极化条件下比较野生类型 (WT) 和FABP5淘汰赛 (KO) BMDM.
- 在不同的酸化位点进行了激酶丰富分析.
主要成果:
- 在M1和M2 BMDM中,FABP5删除显著改变了蛋白质和蛋白表达.
- 受影响的关键途径包括炎症,细胞因子产生,氧化应激和激酶活性.
- 在M1两极化FABP5KO巨细胞中观察到高反应性氧物种 (ROS) 水平,以及GRK家族激酶的变化.
结论:
- FABP5的丧失对极化巨细胞的蛋白质和蛋白质概况产生了深远的影响.
- 删除FABP5影响了对炎症反应和巨细胞功能至关重要的多种途径.
- 这项研究为探索FABP5抑制作为炎症条件的治疗策略奠定了基础.
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