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纤维溶解剂缺陷使宿主易患有导管相关性尿路感染引起的败血症
Jonathan J Molina1,2,3, Kurt N Kohler2,3, Christopher Gager2
1Integrated Biomedical Sciences, University of Notre Dame, IN 46556, USA.
Research square
|October 4, 2023
概括
患有纤维解质缺乏症的患者容易发生严重的导管相关尿路感染 (CAUTIs) 和败血症. *E. faecalis*利用纤维素促进CAUTI病原体的生长和持久性.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 传染性疾病 传染性疾病
- 微生物学 微生物学
背景情况:
- 导管相关的尿路感染 (CAUTIs) 是常见的医院获得的感染,往往导致血液感染和死亡.
- 在CAUTI病原体中的多药耐药性使治疗复杂化.
- 膀中的纤维素积累作为CAUTI病原体生物膜形成的平台.
研究的目的:
- 调查宿主纤维解析能力在CAUTI严重程度和二次败血症中的作用.
- 确定CAUTI病原体,特别是E. faecalis*利用宿主因子促进感染进展的机制.
主要方法:
- 使用CAUTI的小鼠模型.
- 与基因或获得的纤维素分析缺陷的小鼠和对照小鼠的感染结果进行比较.
- 研究了Enterococcus faecalis*分泌的蛋白酶SpreE在纤维素积累和生物膜形成中的作用.
主要成果:
- 宿主纤维解质缺陷会导致严重的CAUTI和败血症,即使是各种尿病原体.
- 导管膀中的纤维素沉积与感染严重程度的增加有关.
- *E. faecalis*利用SpreE诱导纤维素的积累,促进其生长,生物膜的形成,并在CAUTI期间持久.
结论:
- 主体纤维解质状态是CAUTI和败血症风险的关键决定因素.
- 向纤维素积累或细菌蛋白酶 (如SpreE) 可能为CAUTI提供新的治疗策略.
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