用商业仪器生成的微生物单细胞地图
Adam Abate1, Xiangpeng Li1, Linfeng Xu1
1University of California, San Francisco.
Research square
|October 4, 2023
概括
EASi-seq可实现高质量的单个微生物基因组测序,创建详细的微生物群地图. 这种可访问的方法有助于利用先进的生物信息学发现微生物物种亚群.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 生物信息学是一种生物信息学.
背景情况:
- 单细胞测序对于分析复杂的生物系统至关重要,但对微生物缺乏可访问的工具.
- 商业仪器广泛用于哺乳动物单细胞分析,为微生物研究创造了一个空白.
研究的目的:
- 引入EASi-seq (易于访问的单个微生物测序),这是一种用于高效的单个微生物基因组测序的新方法.
- 通过使用单细胞数据,为人类和环境微生物组生成详细的地图.
主要方法:
- 针对微生物应用,调整了Mission Bio Tapestri仪器的单细胞工作流.
- 开发了一个伴随生物信息管道用于集群,组装,菌株识别和微生物基因组的分类.
- 集成的元基因组与EASi-seq数据相结合,以提高捕获偏差的减少和覆盖率.
主要成果:
- 通过EASi-seq,每次运行可以对数千种微生物进行测序,从而产生大型的射击枪基因组数据集.
- 生物信息管道改善了整个基因组组装,菌株识别,分类学分类和基因注释.
- 与元基因组数据的整合成功地减少了捕获偏差和增加了基因组覆盖范围.
结论:
- EASi-seq为从微生物样本中获取单细胞基因组数据提供了高质量的,可访问的工作流.
- 这种方法为发现和分析微生物物种亚群开辟了新的途径.
- EASi-seq利用商业上可用的平台,促进微生物组研究的广泛采用.
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