在种子母基因库中发现有用的遗传变异,以改善
Neeraj Kumar1,2, J Lucas Boatwright1,2, Sirjan Sapkota1
1Advanced Plant Technology, Clemson University, Clemson, SC, United States.
Frontiers in genetics
|October 4, 2023
概括
一个新的多亲先进世代交叉 (MAGIC) B-line种群在中被开发来研究定量特征. 这种遗传资源确定了52种标记特征的关联,包括新型的,有助于开发优异的杂交.
科学领域:
- 植物遗传学和育种.
- 定量性质特征遗传学 遗传学
- 的基因组学 的基因组学
背景情况:
- 多父母种群的遗传多样性比双父母种群的遗传多样性更大,用于研究复杂的特征.
- 一个多亲先进世代交叉 (MAGIC) B-line (MBL) 种群是由代表主要植物品种的四个创始人线创造的.
- 这种MBL群体捕获了重要的遗传变异,对于谷物 (Sorghum bicolor) 的定量特征研究至关重要.
研究的目的:
- 开发和表征一种新的MAGIC B系种群,用于的遗传研究.
- 确定7个影响产量的关键特征的标记特征关联 (MTA).
- 发现可取的等位体组合,以改善的繁殖,特别是B-线.
主要方法:
- 708重组杂交系 (RILs) 在两个位置进行表型化,以检测植物高度,谷物产量和几天到合成等特征.
- 使用多样性阵列技术 (DArTseq) 来生成8,800个单核酸多态 (SNP) 的基因型定型.
- 进行全基因组关联研究 (GWAS) 用3,751个质量过的SNP和表型数据,包括多变量自适应收缩分析.
主要成果:
- 在所有七个特征中都观察到高的表型变异和广义遗传性.
- 对于七个特征,确定了52个MTA,包括与植物高度位置 (Dw1, Dw2, Dw3) 的关联.
- 在Chr1和Chr7上发现了两种新的MTA用于产量特征,独立于矮化基因,15种MTA显示出性效应.
结论:
- MBL种群是剖析的定量特征的宝贵资源.
- 具有理想的等位基组组合的精选后代可以用于开发优异的杂交.
- 新发现的等位基组组合可以通过标记器辅助育种来增强的生殖质.
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