高自模式在膨胀血小板在阿菲雷西斯期间血小板储存 血小板储存
Lu Yu1, Shifang Yu2, Yunlei He1
1The Ningbo Central Blood Station, Ningbo, China.
概括
储存的血小板显示反应性氧物种 (ROS) 和自的增加,特别是在较大的,胀的血小板中. 这表明线粒体ROS和储存期间增强的血小板自之间存在联系.
科学领域:
- 血液银行和输血医疗服务
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 血小板在储存期间表现出显著的形态和功能变化.
- 血小板结构异质性和自之间的关系仍然不太清楚.
研究的目的:
- 在常规储存期间,在体外研究血小板自.
- 探索反应性氧物种 (ROS) 在调节血小板自中的作用.
主要方法:
- 流细胞计测试以评估ROS和自水平.
- 西方涂抹用于测量与自相关的蛋白质 (LC3I,LC3II,Beclin1,帕金,PINK1).
- 传输电子显微镜 (TEM) 用于形态分析.
主要成果:
- 储存的血小板显示总和线粒体ROS的增加.
- 自标志物LC3被放大,与线粒体ROS增加相关.
- 大型,胀的血小板显示较高的LC3水平和增强的自.
- 贝克林1和LC3II/LC3I比率增加,而帕金和PINK1水平保持稳定.
结论:
- 血小板自与储存期间线粒体ROS增加有关.
- 大型,胀的血小板的一个亚群表现出明显的自模式.
- 这些发现表明,在储存期间,胀血小板的自率更高.
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