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Updated: Jul 24, 2026

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确定哺乳动物细胞系中超氧化物脱酶含量和酶活性

Ilzé Engelbrecht1,2, Suranie Horn1,2, John P Giesy3,4,5,6

  • 1Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520, South Africa.

MethodsX
|October 4, 2023
PubMed
概括

这项研究提出了一种简单,具有成本效益的方法,用于测量细胞培养中的抗氧化酶,如超氧化脱酶 (SOD) 和催化酶 (CAT). 这种方法可用于资源有限的环境中的研究人员.

关键词:
有96个井的微板.吸收能力 吸收能力抗氧化酶酶是一种抗氧化酶.细胞培养培养的细胞培养.基于细胞的SOD和CAT生物测试.过氧化的分解分解在体外生物测试.铁醇的自氧化过程用 permanganate 进行定位.

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Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...