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用光学连贯显微镜对人类角膜微结构进行非侵入性in vivo成像

Hadiya F Pattan¹, Xiao Liu¹, Patrice Tankam^1,2

¹School of Optometry, Indiana University, Bloomington, IN, 47405, USA.

Biomedical optics express

|October 4, 2023

概括

一种新的偏振依赖光学连贯显微镜 (POCM) 能够对人类角膜微观结构进行非侵入性,体内成像. 这项技术可以对细胞层进行详细的可视化,用于早期疾病检测和治疗监测.

科学领域:

生物医学光学生物医学光学
眼科医生眼科眼科
医疗成像医学成像

背景情况:

非侵入性成像对于早期的角膜疾病检测和管理至关重要.
需要细胞层次的分辨率来识别微妙的生物标志物.
当前的成像技术可能在分辨率或侵入性方面面临限制.

研究的目的:

开发和验证一种非接触偏振依赖的光学连贯显微镜 (POCM),用于体内人角膜成像.
为了实现角膜微观结构的高分辨率体积成像.
为了克服镜像反射和运动工件的局限性.

主要方法:

在通用路径干扰仪设计中集成的四分之一波板.
使用偏振依赖光学连贯显微镜 (POCM) 系统.
实施了一种自我干扰方法来纠正角膜曲率和运动.

主要成果:

获得了角膜微结构 (内皮细胞) 的体积成像,具有约2.2μm的同位素分辨率.
在0.5x0.5mm^2的视野中以1Hz的频率捕捉到的图像.
成功地可视化了前角膜层,包括上皮细胞和状角细胞.

结论:

开发的POCM系统提供了人类角膜的非侵入性,高分辨率的体内成像.
这项技术有可能用于眼科早期诊断,疾病监测和治疗评估.
POCM的进步促进了更深层次的组织透和人工物减少,用于细胞水平的角膜成像.

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