测量表面张力和计算模型生物分子凝聚物
Jack Holland1,2, Alfonso A Castrejón-Pita3, Remco Tuinier4
1Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, OX1 3QZ, UK. dirk.aarts@chem.ox.ac.uk.
Soft matter
|October 4, 2023
概括
我们开发了一种简单的方法,使用滴分析测量生物分子凝聚物的表面张力. 发现增加的蛋白质净电荷降低了凝结物的表面张力,为细胞组织提供了洞察力.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 软物质物理学 软物质物理学
背景情况:
- 生物分子凝结物是细胞内的类似液体的结构.
- 它们的表面张力是影响细胞组织的关键物理性质.
- 以前测量表面张力的方法很复杂,需要粘度数据.
研究的目的:
- 介绍一种简单的方法来评估模型生物分子凝聚物的表面张力.
- 为了研究蛋白质特性对凝结物的表面张力的影响.
- 评估理论模型在预测凝结物表面张力的应用性.
主要方法:
- 毛细血管长度和凝结物密度的滴测量.
- 使用的模型缩物是由内在无序的蛋白Ddx4N突变物制成的.
- 应用了Scheutjens-Fleer理论与Flory-Huggins相互作用参数.
主要成果:
- 开发了一种简化的表面张力测量方法,绕过粘度表征.
- 证明Ddx4N蛋白质的净电荷增加会对凝结物表面张力产生负面影响.
- 施欧廷斯-弗莱尔理论在预测表面张力方面提供了半定量准确性.
结论:
- 这种新方法提供了一种更简单的方法来研究生物分子凝结物的表面张力.
- 蛋白质净电荷是调节凝结物表面张力的关键因素.
- 理论建模可以提供对凝结体界面的分子组织的见解.
相关概念视频
01:22Surface Tension of Fluid
313
Surface tension is a fundamental property of fluids, occurring at the boundary between a liquid and a gas or between two immiscible liquids. This phenomenon arises from the cohesive forces between molecules at the fluid's surface, creating an effect similar to a stretched elastic membrane. Inside each fluid, molecules are equally attracted in all directions by neighboring molecules, but surface molecules experience a net inward force, resulting in surface tension.
Surface tension varies...
Surface tension varies...
313
02:57Surface Tension, Capillary Action, and Viscosity
28.0K
Surface Tension
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...
28.0K
01:16Surface Tension and Surface Energy
1.4K
When a paint brush is immersed in water, the bristles wave freely inside the water. When it is taken out, the bristles stick together. The reason behind this effect is surface tension.
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
1.4K