卡洛巴克特的NtrB-NtrC两组系统弥合了同化和细胞发育的桥梁
Hunter North1, Maeve McLaughlin1, Aretha Fiebig1
1Department of Microbiology and Molecular Genetics, Michigan State University , East Lansing, Michigan, USA.
Journal of bacteriology
|October 4, 2023
概括
细菌NtrC蛋白调节了Caulobacter中的同化和细胞发育. 它的缺失会影响增长,但IS元素的转换可以挽救这些缺陷,影响进化.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 分子生物学分子生物学
背景情况:
- 考洛巴克特利用分子感官系统,根据营养的可用性来调节生长和发育.
- 在许多细菌中,NtrB-NtrC双组分系统对于同化至关重要,但其在Caulobacter中的特定作用尚未完全理解.
- 考洛巴克特的NtrC是一种非常规的细菌增强剂结合蛋白 (bEBP),缺乏典型的σ54相互作用的GAFTGA基因.
研究的目的:
- 研究NtrB和NtrC在Caulobacter crescentus代谢和发育中的功能.
- 为了确定NtrC结合于Caulobacter染色体的直接目标.
- 建立NtrC,代谢和细胞发育过程之间的监管联系.
主要方法:
- 在Caulobacter crescentus中删除ntrC和ntrB基因的突变性.
- 在各种源下分析细胞生长.
- 使用ChIP-seq识别NtrC结合点,并分析IS元素的转换.
- 评估极地茎的延长和细胞外多糖体的合成.
主要成果:
- 删除ntrC减缓了Caulobacter的生长,ntrB/ntrC对于的生长至关重要,因为它们在谷氨胺合成酶表达中的作用是唯一的源.
- IS3家族元素的自发转换经常通过恢复glnBA操作子转录来挽救ntrC突变的生长缺陷.
- 确定了数十个直接的NtrC结合位点,其中许多靠近多糖生物合成基因,并与GapR和MucR1.1的结合位点重叠.
- 丧失NtrC功能导致长长的极茎和增加细胞外多糖化合物合成.
结论:
- 在Caulobacter的同化,细胞循环调节和极性形态发生过程中,NtrC起着重要作用.
- 转化IS3元素可以作为一个进化机制来克服由ntrC突变引起的营养不足.
- 通过调节参与多糖合成的基因,NtrC直接影响细胞发育,并与关键细胞循环蛋白共享调节作用.
关键词:
这是一种Caulobacter.细胞循环中的细胞循环.增强剂-结合蛋白质增强剂-结合蛋白质这就是的酸.核子中的核子.营养物质的吸收和同化西格玛因子 (Sigma Factor) 是一个因子.信号传导的信号传导.转录规则 转录规则 转录规则更多相关视频
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