温度波动调节骨肌肉中的分子机制,并影响牛肉的生长潜力
Zachary K Smith1, Erika Eckhardt2, Won Seob Kim2
1Department of Animal Science, South Dakota State University, Brookings, SD, USA.
Journal of animal science
|October 4, 2023
概括
温度波动导致肉牛的热应激,影响肌肉生长和脂肪积累. 这项研究揭示了运输过程中细胞对热应激的反应,突出了关键的蛋白质和基因表达变化.
科学领域:
- 动物科学动物科学
- 生理学 生理学 生理学
- 生物技术是生物技术.
背景情况:
- 温度波动对牲畜福利和生产力构成重大挑战.
- 了解热应激的细胞和生理反应对于减轻对肉牛的负面影响至关重要.
研究的目的:
- 为了研究严重的温度波动对红斯牛肉牛的细胞和生理反应的影响.
- 确定热应激的分子标记物及其对骨肌肉和脂肪组织发育的影响.
主要方法:
- 18个牛肉牛被分为三个组:控制 (CON),运输 (TP) 和温度波动 (TS).
- 在一英里的拖车行程中,TS组的温度湿度指数 (THI) 从42到72-75发生了变化.
- 基因和蛋白质表达分析 (HSP,肌肉生长和脂肪生成因素) 在长极肌肉样本上进行.
主要成果:
- 温度波动 (TS) 诱导了可观察到的热应激行为,包括食欲下降和呼吸速率增加.
- 在TS治疗导致显著过度表达热冲击蛋白 (HSPs) 20,27,和90在长肌.
- 在TS组中观察到骨肌肉生长标志物 (Pax7,Myf5,MHC) 和脂肪生成转录因子 (PPARγ,C/EBPα,FAS,SCD) 的上调.
结论:
- 严重的温度波动,即使没有恒定的高温,也会导致肉牛的热应激.
- 这些波动会影响调节骨肌肉生长和脂肪组织积累的细胞机制.
- 调查结果表明,由于运输引起的热应激,肉质和尸体成分可能发生变化.
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