类风湿性关节炎临床因素与突细胞类型和状态之间的关联
Dana Weisenfeld1, Fan Zhang1,2,3, Laura Donlin4
1Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts.
Arthritis & rheumatology (Hoboken, N.J.)
|October 4, 2023
概括
类风湿性关节炎 (RA) 疗法,而不是临床因素,显著影响突细胞类型. 了解这些细胞变化是预测RA患者治疗反应的关键.
科学领域:
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
- 基因组学就是基因组学.
背景情况:
- 类风湿性关节炎 (RA) 突表现出多种细胞类型和状态.
- 有限的数据将突细胞发现与患者级临床信息相关联.
研究的目的:
- 确定RA临床因素与突细胞类型/状态之间的关联.
- 利用最大的队列,结合临床和多细胞数据进行RA研究.
主要方法:
- 招募了活跃的RA患者 (DMARD-naive或不充分响应者).
- 收集了RA临床因素,并进行了突细胞活检.
- 采用转录组和表观素序列的细胞索引 (CITE-seq) 来获得细胞类型百分比和CTAP (例如,EFM,TF).
- 与细胞类型百分比和CTAP相关的RA临床因素.
主要成果:
- 较高的DAS28-CRP3得分与增加的T细胞百分比相关.
- 与没有服用DMARD的患者相比,接受甲醇 (MTX),但不是生物DMARD (bDMARD) 的患者的B细胞百分比较高.
- 接受bDMARDs的患者主要表现出内皮细胞,纤维细胞和髓状细胞 (EFM) 细胞表型,而T细胞和纤维细胞 (TF) 类别中没有.
结论:
- 与其他临床因素相比,DMARDs在RA中显示与突细胞表型的差异性关联.
- RA 疗法可能会影响突细胞类型/状态,可能会影响随后的治疗反应.
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