通过调节Atoh7,TRIM9促进来自Müller细胞的视网膜干细胞通过调节Atoh7来分化为视网膜质细胞

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Qi Zeng¹, Jinglin Zhou², Xingyu Hua²

¹Department of Ophthalmology, Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, Furong District, Changsha, 410008, China. zengqi1216@hunnu.edu.cn.

In vitro cellular & developmental biology. Animal

|October 4, 2023

概括

三方基因含有蛋白9 (TRIM9) 促进了穆勒细胞分化成视网膜质细胞 (RGCs),这对于治疗青光瘤至关重要. 这通过调节Atoh7表达而发生,为这种致盲眼病提供了潜在的治疗途径.

科学领域:

眼科医生眼科眼科
神经科学是一个神经科学.
干细胞生物学干细胞生物学

背景情况:

玻璃眼瘤是一种不可逆转的致盲眼病,导致视网膜质细胞 (RGC) 死亡.
来自穆勒细胞的视网膜干细胞 (RSC) 显示了RGC再生的潜力.
了解调节RSC差异化的因素是治疗青光眼的关键.

研究的目的:

研究TRIM9在Müller细胞分化成RGC中的作用和机制.
探索TRIM9作为治疗眼的治疗点的潜力.

主要方法:

建立了一个高眼内压 (IOP) 鼠标模型,使用表皮静脉烧结.
隔离和识别了Müller细胞,诱导它们去分化为RSCs.
在RSC中使用了lentiviral TRIM9过度表达和Atoh7敲击用于体外研究.

主要成果:

在高IOP模型中,TRIM9被上调;米勒细胞表现出异常的增殖.
在RSC中TRIM9的过度表达促进了神经元外生和增加RGC标记物表达 (Brn-3b,Thy1.1).
TRIM9的亲差异化效应依赖于Atoh7,因为敲击扭转了变化.

结论:

TRIM9增强了Müller细胞分化成RGCs,可能通过Atoh7调节.
TRIM9代表了一种有前途的治疗点,用于促进青光眼中RGC再生.

关键词:

眼光障碍症是什么眼光障碍症是什么穆勒细胞是什么意思?视网膜质细胞视网膜质细胞三方图案包含9个包含9个

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