黄素通过caspase 3促进人类黑色素瘤细胞的亡
Daiane Manica1, Gilnei Bruno da Silva2, Alana Patrícia da Silva1
1Postgraduate Programme in Biochemistry, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Cell biochemistry and function
|October 4, 2023
概括
黄素是一种天然化合物,通过诱导亡,有效地降低了转移性皮肤黑色素瘤 (CM) 细胞的活力和迁移. 这项研究证明了黄素的存在.
科学领域:
- * 药理学 药理学 药理学
- * 瘤学 在线咨询
- * 自然产品 化学 化学
背景情况:
- *皮肤黑色素瘤 (CM) 是一种转移性癌症,治疗选择有限.
- *自然化合物,如多,显示承诺作为CM的辅助疗法.
- *黄素的抗瘤作用需要在先进的CM中进行研究.
研究的目的:
- *为了评估黄素对转移性CM细胞系SK-MEL-28.8.的影响.
- * 调查黄素对细胞活力,细胞亡,迁移和氧化还原平衡的影响.
- * 探索黄素对CM细胞中酶3激活的影响.
主要方法:
- *MTT测定和光显微镜用于细胞活力.
- * Annexin V 流细胞测量用于检测细胞亡.
- *用于评估细胞迁移的伤口愈合试验.
- *测量反应性氧物种 (ROS),氧化 (NOx) 和硫醇.
- *基因表达和酶活性测定caspase3的测定.
主要成果:
- *黄素显著降低了SK-MEL-28细胞活力,并抑制了细胞迁移.
- *黄素在较高度和增加ROS和NOx水平时诱导了亡.
- *黄素提高了caspase 3的基因表达和酶活性.
- *黄素促进氧化应激,激活酶通路,导致CM细胞死亡.
结论:
- *黄素对皮肤黑色素瘤细胞表现出显著的抗转移作用.
- *黄素诱导的氧化应激和caspase 3激活是其主要的作用机制.
- *黄素具有作为高级皮肤黑色素瘤的辅助治疗剂的潜力.
更多相关视频
08:41Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
Published on: January 12, 2016
11.8K
09:18Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
Published on: December 27, 2016
8.7K
相关概念视频
01:24Caspases
12.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
12.6K
01:31The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
01:17The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
01:30Apoptosis
11.6K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.6K
01:17Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K