将APEX2定位为酵母中的编码脂肪酸合成酶β的mRNA,可以识别相互作用的蛋白质,控制其在细胞周期中的丰度

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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
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Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

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Heidi M Blank1, Wendell P Griffith2, Michael Polymenis1

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843.

Molecular biology of the cell
|October 4, 2023
PubMed
概括

研究人员使用近距离标记在细胞周期期间识别了与FAS1mRNA相互作用的蛋白质. 发现糖分酶Tdh3p对于Fas1p是必不可少的.

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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