对牙口腔癌单细胞转录组分析揭示了两个主要的细胞程序
Sillarine Kurkalang1, Sumitava Roy1,2, Arunima Acharya1,2
1National Institute of Biomedical Genomics, Kalyani, India.
Cancer science
|October 4, 2023
概括
这项研究揭示了牙口腔状细胞癌 (OSCC-GB) 的细胞多样性,识别了不同的细胞状态和免疫反应. 这些发现突显了口腔下粘膜纤维化 (OSMF) 瘤中的关键分子差异.
科学领域:
- 在瘤学瘤学.
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 牙口腔状细胞癌 (OSCC-GB) 是印度流行的一种癌症,结果不佳.
- 了解细胞异质性对于改善OSCC-GB预后和治疗至关重要.
研究的目的:
- 用单细胞RNA测序来研究OSCC-GB中的细胞异质性.
- 在患有口腔下粘膜纤维化 (OSMF) 的瘤中识别独特的恶性细胞类型和树皮细胞表型.
- 探索OSCC-GB瘤微环境中的免疫细胞透和抗瘤反应.
主要方法:
- 来自12名OSCC-GB患者瘤的单细胞RNA测序.
- 对具有并发OSMF和没有并发OSMF的瘤的分析.
- 对基因表达进行分析,以识别歧视性标记物 (FOS,ATP1A,DUSP1).
- 免疫细胞种群及其状态的表征.
主要成果:
- 在OSCC-GB与OSMF中确定了明显的恶性细胞类型和树皮细胞表型变化.
- FOS,ATP1A和DUSP1的表达水平在有或没有OSMF的瘤中差异化.
- 恶性细胞表现出部分上皮层-介质细胞过渡 (pEMT) 或胎儿细胞重编程特征.
- 与免疫相关的通路基因在恶性细胞中得到丰富,使用胎儿/pEMT程序.
- 观察到免疫细胞透率的增加,多种T细胞亚型,以及M1-M2巨细胞的中间两极分化.
结论:
- OSCC-GB表现出显著的细胞异质性,与与OSMF存在相关的独特细胞状态.
- 在OSCC-GB恶性细胞中,pEMT和胎儿细胞重编程是主要的程序.
- 瘤微环境的特点是大量的免疫细胞透和特定的免疫细胞亚型,表明有活跃的抗瘤反应.
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