在HUVEC中的ROS生产和SOD活动中的种族和性别差异
Sara E Mascone1, Katherine I Kim1, William S Evans1
1Department of Kinesiology, School of Public Health, University of Maryland, College Park, MD, United States of America.
PloS one
|October 4, 2023
概括
黑人和白人男性和女性对炎症表现出不同的反应. 男性细胞表现出更高的反应性氧物种 (ROS) 和促炎状态,而女性细胞表现出适应性超氧化物转化酶 (SOD) 活性.
科学领域:
- 内皮细胞生物学 内皮细胞生物学
- 心血管研究的心血管研究.
- 炎症和氧化应激作用的情况.
背景情况:
- 高血压的患病率因种族和性别而异.
- 黑人和男性容易患上更早,更严重的高血压.
- 了解这些差异背后的细胞机制至关重要.
研究的目的:
- 调查种族和性别对反应性氧物种 (ROS) 生产和超氧化物转化酶 (SOD) 活性的影响.
- 为了评估人类静脉内皮细胞 (HUVECs) 的细胞反应,来自黑人和白人个体,按性别分层,在诱导炎症后.
主要方法:
- 来自黑人和白人男性和女性的HUVEC被培养.
- 使用瘤缩因子-α (TNF-α) 诱导了炎症.
- 在基线和TNF-α暴露24小时后测量了ROS产量和SOD活性.
主要成果:
- 黑色和白色HUVEC之间的ROS生产或SOD活性没有显著差异.
- 女性HUVEC的ROS产量明显低于男性HUVEC.
- 与女性HUVEC相比,男性HUVEC表现出一种促炎状态.
- 与对照人群相比,白色HUVEC在TNF-α治疗下显示出淡的SOD活性.
- 雌性HUVEC在长时间暴露于TNF-α时显示出SOD活性增加.
结论:
- 种族对HUVECs的ROS产量或SOD活性没有显著影响.
- 性别显著影响炎症反应,男性的HUVEC显示出更大的氧化应激.
- 雌性HUVEC表现出适应性SOD对炎症的反应,这表明了潜在的保护机制.
- 这些发现突出了炎症反应中的性别特异细胞差异,导致高血压差异.
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