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活细胞成像揭示了组 histone 乙化,转录启动和核细胞的移动性之间的关系

Matthew N Saxton¹, Tatsuya Morisaki¹, Diego Krapf²

¹Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.

Science advances

|October 4, 2023

概括

基因组乙化 (H3K27ac) 和RNA聚合酶II酸化 (RNAP2-Ser5ph) 标志着不同的染色质状态. 这些修改通常是单独的,具有相反的动态,表明平衡与活跃转录中的作用.

科学领域:

分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
染色体动力学染色体动力学

背景情况:

基因乙化和RNA聚合酶II酸化是与基因转录相关的关键表观遗传标记.
活细胞中这些修饰的精确时空关系和动态尚不清楚.
了解这些动态对于破译转录调节机制至关重要.

研究的目的:

在活细胞中,研究基因组H3 lysine-27乙化 (H3K27ac) 和RNA聚合酶II serine-5酸化 (RNAP2-Ser5ph) 之间的空间和时间关系.
量化这些特定修改丰富的染色体区域的动态.
阐明转录活性或平衡色素中这些修饰的功能意义.

主要方法:

利用基于Fab的内源蛋白质修饰的标签,以精确检测.
采用单分子追踪技术,以高分辨率监测染色质动态.
在由H3K27ac和RNAP2-Ser5ph标记的不同的染色体环境中,量化核细胞扩散率.

主要成果:

发现富含H3K27ac和RNAP2-Ser5ph的染色体区域通常在空间上是分离的.
这两种修改在分钟时间尺度上表现出反向相关性.
在次秒时间表上,核细胞在H3K27ac丰富的染色体中扩散速度约为15%,在RNAP2-Ser5ph丰富的染色体中扩散速度约为15%.

结论:

高水平的H3K27ac和RNAP2-Ser5ph通常不会在同一地点和时间共存.
H3K27ac和RNAP2-Ser5ph可能标志着染色质的不同状态:分别处于静止状态和积极转录状态.
不同的核细胞体动力学支持这些表观遗传标记在转录调节中的独特作用.

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