血生成的氧化水:一种有前途的策略,用于对抗环境污染物微型菌的细菌休眠状态 (VBNC状态)
Shweta B Borkar1, Manorma Negi1, Apurva Jaiswal1
1Department of Electrical and Biological Physics/Plasma Bioscience Research Center, Kwangwoon University, Seoul 01897, South Korea.
Journal of hazardous materials
|October 4, 2023
概括
血生成的氧化水 (PG-NOW) 有效地消除了微型白球菌,防止了可活但不能培养 (VBNC) 状态. 这表明PG-NOW是控制细菌污染和公共卫生风险的有希望的工具.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 公共卫生 公共卫生
背景情况:
- 可生存但不能培养 (VBNC) 状态是细菌在压力下生存的机制.
- 由于潜在的复苏,VBNC细菌对食品安全和公共卫生构成重大风险.
- 控制细菌污染和防止VBNC形成至关重要.
研究的目的:
- 为了研究血产生的氧化水 (PG-NOW) 对空气传播的微型白杆菌 (M. luteus) 的疗效.
- 为了确定PG-NOW治疗是否会在M. luteus中诱导VBNC状态.
- 评估PG-NOW在防止VBNC形成方面的潜力.
主要方法:
- 对M. luteus暴露于PG-NOW. 的情况.
- 培养方法来评估细菌的生存能力.
- 酸单化物定量PCR (PMAxx-qPCR) 用于区分活细胞和死细胞.
- 对复苏相关因素的基因表达分析.
主要成果:
- PG-NOW有效地消除了M. luteus,幸存的细菌仍然可以培养.
- 使用联合培养和PMAxx-qPCR方法没有观察到显著的VBNC诱导.
- 基因表达分析揭示了促进复苏的因素,氨基酸和脂肪酸代谢基因的上调.
- 接受PG-NOW治疗的M. luteus显示肺细胞感染能力降低.
结论:
- PG-NOW有效地禁用M. luteus,并且不会诱导VBNC状态.
- PG-NOW显示出控制细菌污染和防止VBNC形成的潜力.
- 这些发现为使用PG-NOW反应性物种进行微生物控制提供了洞察力.
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