用两个样本来进行血液THC度逆向推断的方法 - - 这是可行的吗?
Peder Olai Skjeflo Holman1, Gudrun Høiseth2, Liliana Bachs2
1Department of Forensic Sciences, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway; Department of Pharmacology, Oslo University Hospital, PO Box 4950 Nydalen, 0424 Oslo, Norway.
Forensic science international
|October 4, 2023
概括
血液delta-9-tetrahydrocannabinol (THC) 的逆向抽取是不可靠的. 由于药物动力学复杂性和测量错误,两份分隔不久的血液样本不足以准确确定THC水平.
科学领域:
- 法医毒理学 法医毒理学
- 药理动力学 药理动力学
- 分析化学 分析化学
背景情况:
- 血液中药物度的逆向抽取对于驾驶障碍病例至关重要.
- 德尔塔-9-四大麻 (THC) 呈现出复杂的药理动力学,使血液度抽取复杂化.
- 这项研究评估了一种逆向抽取方法,使用两个血液样本来估计THC消除率.
研究的目的:
- 为了评估血液THC度的逆向抽取值的可靠性.
- 为了确定两个连续的血液样本是否可以准确地估计被捕司机的THC消除率.
- 分析抽样时间和测量不确定性对THC推算精度的影响.
主要方法:
- 利用了挪威 (2000-2020年) 548名被逮捕的司机的数据,其中两个THC血液样本分隔≥20分钟.
- 使用GC-MS和UHPLC-MS/MS测量了THC度.
- 从THC度和时间间隔 (Δt) 估计了速率常数 (k),用线性回归来建模关系.
主要成果:
- 样本间的中位时间 (Δt) 为31分钟;血液中位THC为2.4μg/L (第一样本) 和2.3μg/L (第二样本).
- 在62%的病例中,THC度下降,但由于测量不确定性,在87%的病例中没有统计学意义.
- 计算的明显半衰期 (t1⁄2) 为6.0小时,回归预测的t1⁄2从0.93到13小时不等.
结论:
- 计算的THC半衰期 (6.0小时) 比预期的要短,这表明样品是在分发阶段采集的.
- 短的采样间隔 (Δt) 和分析错误掩盖了真正的药理动力学变化,使单个k值变得不可靠.
- 由于固有的不可靠性,不建议采用两样样本的方法来进行THC的逆向抽取.
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