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在高度敏感的心脏移植患者中,IgM标记着持久的IgG抗人类白细胞抗原抗体

Fang Li¹, Loren Gragert², D Giovanni Biagini²

¹Comprehensive Transplant Center, Cedars-Sinai Medical Center, Los Angeles, California.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation

|October 4, 2023

概括

免疫球蛋白M (IgM) 抗体增强了心脏移植候选人的免疫球蛋白G (IgG) 抗人类白细胞抗原 (HLA) 抗体的强度和持久性. 这种相互作用可能会指导高度敏感的患者选择捐赠者.

科学领域:

免疫学免疫学免疫学
移植科学移植科学
血清学血清学是一门学科.

背景情况:

人类白细胞抗原 (HLA) 敏感化对心脏移植 (HT) 接受者构成重大挑战.
了解高度敏感 (HS) 的HT候选人中抗HLA抗体和B细胞库的特征至关重要.

研究的目的:

在HSHT候选人中表征免疫球蛋白G (IgG) 和免疫球蛋白M (IgM) 抗HLA抗体库.
研究HS候选人的抗HLA抗体和B细胞受体谱序列测序 (BCRseq) 之间的关联.

主要方法:

在11名HS和3名轻度敏感 (MS) 候选人中使用Luminex单抗原珠测定评估IgG和IgM抗HLA抗体.
在HS候选和33个非候选控制中执行了BCRseq.
使用平均光强度 (MFI) 测量HLA抗体强度.

主要成果:

在所有HS候选人中都存在IgM抗HLA抗体,其宽度和强度低于IgG.
交叉的IgM和IgG抗HLA特异性显示出更高的结合强度.
抗HLA抗体的IgG降低了HT后,如果IgG与移植前的IgM交叉,强度下降较小.

结论:

存在IgM表明IgG抗HLA抗体的强度和移植后持久性更高.
识别IgM交叉IgG抗HLA特异性可以有助于为敏感的HT候选人选择相容的捐赠者.

关键词:

在 HLA 敏感化过程中,所有的抗体.抗体的异型为抗体的同型.心脏移植心脏移植手术人类白细胞抗原.拒绝拒绝拒绝拒绝

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