相关实验视频

Updated: Jul 15, 2025

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

来自Cantharellus yunnanensis的多糖体的结构特征和体外低血糖活性

Run Tian¹, Yu-Zhuo Zhang², Xianbo Cheng³

¹Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Provincial Key Laboratory for Research and Development on Tropical Herbs, School of Pharmacy, Hainan Medical University, Haikou 571199, China; Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou 571158, China.

International journal of biological macromolecules

|October 4, 2023

概括

来自C. yunnanensis的新型多糖体CY-2显示出作为降糖剂的巨大潜力. 它有效地抑制α-葡萄糖酶,并改善细胞中的葡萄糖代谢,表现优于甲福林.

科学领域:

植物化学植物化学
生物化学生化学
药理学药理学是指药理学的学科.

背景情况:

多糖因其多样化的生物活动而越来越受认可.
探索用于治疗应用的新型植物化合物对于药物发现至关重要.
多糖的低血糖潜力需要进一步研究.

研究的目的:

从C. yunnanensis.中分离和描述一种新型的多糖体 (CY-2).
评估CY-2的α-葡萄糖酶抑制活性.
评估CY-2在改善HepG2细胞葡萄糖消耗和新陈代谢方面的疗效.

主要方法:

通过提取水,酒精沉和DEAE-52染色学来分离多糖.
使用红外 (IR) 光谱,甲基化分析和核磁共振 (NMR) 的结构阐明.
用HepG2细胞进行α-葡萄糖酶酶抑制试验和基于细胞的试验.

主要成果:

聚糖CY-2被分离出来,其分子量为2.69 × 104 Da,主要由葡萄糖和曼诺糖组成.
结构分析表明一个 →6) -α-D-Manp-(1→3) -β-D-Glcp-(1→ 的骨干, β-D-Glcp-(1→ 作为一个分支链.
在HepG2细胞中,CY-2表现出显著的α-葡萄糖酶抑制和增强的葡萄糖代谢,超过10μg/mL的甲福明.

结论:

CY-2是一种有前途的低血糖药物,具有潜在的治疗价值.
这项研究阐明了CY-2在α-葡萄糖酶抑制中的结构-活性关系.
在细胞模型中,CY-2表现出优异的降血糖效果,与甲福明相比.

关键词:

低血糖的影响低血糖效应物理化学特性物理化学特性结构阐明结构阐明

更多相关视频

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Isolation and Purification of Fungal β-Glucan as an Immunotherapy Strategy for Glioblastoma

Published on: June 2, 2023

相关概念视频

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

Oral Hypoglycemic Agents: α-Glucosidase Inhibitors

α-glucosidase inhibitors, including acarbose (Precose), miglitol (Glyset), and voglibose (Voglib) (primarily available in Asia), are drugs that control blood sugar levels by delaying the digestion of starch and disaccharides. They achieve this by inhibiting α-glucosidase enzymes in the intestine, which slow the absorption of carbohydrates in the intestine, which in turn leads to a prolonged release of the glucoregulatory hormone GLP-1 from intestinal L-cells.
Acarbose and miglitol are...

Chemistry of Carbohydrates

Chemistry of Carbohydrates

Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...