非洲毒素M1降低了编码紧结蛋白的基因的表达,并影响了肠道上皮质的完整性
Lal Krishan Kumar1, Surya Kant Verma1, Rajeev Chandel1
1Molecular Endocrinology, Functional Genomics & System Biology Laboratory, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal (Haryana), India, 132001.
Mycotoxin research
|October 4, 2023
概括
牛奶中的阿弗拉托辛M1 (AFM1) 不会改变细胞的外观或生存,但会破坏肠道屏障基因,可能导致肠道泄漏. 这种真菌毒素通过影响紧密结合的完整性而影响肠道健康.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 胃肠病学 胃肠病学
背景情况:
- 非洲毒素M1 (AFM1) 是一种由牛奶传播的真菌毒素,与细胞损伤有关.
- 肠道屏障的完整性对肠道健康和预防全身毒性至关重要.
研究的目的:
- 为了研究AFM1对人类肠道Caco-2细胞的急性细胞毒性作用.
- 评估AFM1对细胞形态,活力,氧化应激和基因表达的影响.
主要方法:
- 卡科-2细胞在6,12和24小时内暴露在不同的AFM1度 (5-2000 ng/L) 中.
- 试验包括细胞形态,活力,ROS生产 (DCFDA),基因表达 (qPCR) 和透孔透性.
- 分析了与细胞骨和紧结相关的特定基因.
主要成果:
- AFM1没有影响Caco-2细胞形态,但在12小时后在>1000 ng/L下降了10%的活力.
- 在6小时后,观察到活性氧物种 (ROS) 生产增加.
- AFM1改变了基因表达,减少了紧结蛋白 (Claudin-1,Occludin,ZO-1) 和增加了其他蛋白质 (Cytokeratin,Villin,Vimentin,JAM-1,Cyp1a1).
结论:
- 牛奶中的AFM1污染不会明显损害肠道细胞形态或活力.
- 暴露在AFM1下降了关键的肠道屏障转录,这表明破坏肠道屏障功能并促进"漏肠"的机制.
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