在mRNA抗体药物介导的被动免疫疗法的机制和研究进展
Yuxiang Zhao1, Linchuan Gan1, Dangjin Ke1
1Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, College Town, Fuzhou, Fujian, PR China.
Journal of translational medicine
|October 4, 2023
概括
使者RNA (mRNA) 传递系统为抗体生产提供了一种新的方法,克服了传统方法的局限性. 这项技术可以在体内产生抗体,从而降低治疗应用的成本和生产时间.
科学领域:
- 生物技术和制药科学 生物技术和制药科学
- 分子和细胞生物学分子和细胞生物学
- 免疫学和医学疗法
背景情况:
- 传统的抗体生产涉及复杂的体外重组蛋白表达,漫长的周期和严格的储存要求.
- 这些局限性阻碍了抗体疗法的广泛应用,特别是在临床环境中.
- 使者RNA (mRNA) 传递系统为克服这些挑战提供了一个有希望的替代方案.
研究的目的:
- 与传统的体外方法相比,审查基于mRNA的抗体生产的优缺点.
- 阐明mRNA抗体在治疗传染病和癌症方面的进展和潜力.
主要方法:
- 编码抗体的mRNA被封装在纳米材料中,以便有针对性地输送到患者的细胞质中.
- 在分娩时,mRNA被核糖体翻译,随后进行翻译后的修改,以在体内产生功能性抗体.
- 将mRNA抗体生产与传统的体外表达抗体制造工艺进行比较.
主要成果:
- mRNA抗体技术促进了即时的体内抗体生产,消除了复杂的体内表达步骤.
- 这种方法延长了治疗性蛋白质的半衰期,并大大降低了生产成本和时间表.
- 在临床前或临床研究中证明的mRNA抗体的成功应用,用于传染病预防和瘤学.
结论:
- mRNA抗体技术为传统抗体生产提供了一种简化且具有成本效益的替代方案.
- 通过mRNA进行体内抗体的产生对推进生物医学和临床疗法具有重大前景.
- 需要进一步的研究和开发,才能充分发挥mRNA抗体在疾病预防和治疗方面的潜力.
相关概念视频
01:29Microorganisms in Medicine and Therapeutics
37
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
37
01:31Hybridoma Technology
14.9K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
14.9K
01:31Active versus Passive Immunity
670
Immunity, along with the ability to limit pathogen growth to prevent significant body tissue damage, can be gained either by (1) actively developing an immune response within the individual after exposure to a pathogen or after getting vaccinated or (2) passively transferring immune components from an immune individual to one who is nonimmune. Both these forms of immunity can be found naturally and in medical practices.
Active Immunity
Active immunity refers to the resistance one develops...
Active Immunity
Active immunity refers to the resistance one develops...
670