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对人类胎盘的DNA甲基化阵列研究应用epiphenotyping方法

A Khan^1,2,3,4, A M Inkster^1,2, M S Peñaherrera^1,2

¹BC Children's Hospital Research Institute (BCCHR), 950 W 28th Ave, Vancouver, BC, V5Z 4H4, Canada.

Epigenetics & chromatin

|October 4, 2023

概括

使用表现型化工具进行胎盘DNA甲基化分析,准确估计妊娠年龄和祖先. 这些工具有助于计算DNA甲基化数据中的技术和生物因素,改善跨研究的分析.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.
围产期生物学围产期生物学

背景情况:

胎盘的全基因组DNA甲基化 (DNAme) 分析对于了解子宫暴露和胎儿发育至关重要.
技术和生物因素可能会混DNAme变异分析,需要强大的解释方法.
型化方法从DNAme数据中推算出表型变量,有助于跨队列比较和理解DNAme变异性.

研究的目的:

评估表现型工具对胎盘DNA甲基化数据的准确性和实用性.
研究胎盘表现型与技术/生物变量之间的关系.
开发一个处理和分析胎盘DNA甲基化数据的框架.

主要方法:

利用了来自3个队伍的204个胎盘的DNA甲基化数据.
应用了PlaNET R包来估计妊娠年龄,祖先和细胞组成的表现型.
与独立标记物相关联的表现型估计和DNA甲基化变异的分析驱动因素.

主要成果:

PlaNET祖先估计与独立标记有很强的相关性;表观遗传妊娠年龄在4天内准确.
细胞组成因队列和处理时间而异;细胞质细胞的比例随着妊娠年龄而降低.
起源队列和细胞质细胞的比例是DNA甲基化变异的主要驱动因素.

结论:

证实了在DNA甲基化分析中考虑队列,批量,细胞类型,种族,祖先和性别的重要性.
演示表现的表现型化工具提供了对临床数据的独立检查,并允许进行强大的跨数据集比较.
介绍了胎盘DNA甲基化数据处理和分析的框架,其中包含了表现型变量.

关键词:

他们的祖先的祖先.细胞组成细胞组成通过DNA甲基化.表观遗传年龄表观遗传年龄表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.显现型定位 (Epiphenotyping) 是指表现型定位的一个方法.怀孕年龄怀孕年龄这就是PlaNET R包.胎盘胎盘胎盘胎盘

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