甘:在Chaetothyriales黑酵母的一个意想不到的息地
Flávia de F Costa1, Rafael S C de Souza2, Morgana F Voidaleski3
1Engineering Bioprocess and Biotechnology Post-Graduation Program, Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Paraná, Brazil.
IMA fungus
|October 4, 2023
概括
包括人类病原体在内的黑酵母在甘上被发现,使用了转基因组学和隔离. 这项研究发现了新的物种和第一个环境Cladophialophora bantiana,致命的大脑疾病的原因.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 环境微生物学 环境微生物学
背景情况:
- 甘 (Saccharum officinarum) 在经济上对糖和生物燃料很重要.
- 甘是多种多样的内菌和菌的息地.
- 黑酵母 (Herpotrichiellaceae) 居住在污染的环境中,可以引起人类的感染.
研究的目的:
- 评估与甘相关的Herpotrichiellaceae真菌的多样性.
- 使用in silico和in vitro方法识别黑酵母类真菌.
- 从甘及其根球中发现新的真菌物种.
主要方法:
- 超基因组测序用于识别真菌DNA.
- 从甘中选择性地分离黑酵母类真菌.
- 在基分析的真菌序列.
- 在体外培养和分离物的识别.
主要成果:
- 确定了5833个真菌序列,并分离了639个类似黑酵母菌的殖民地.
- 检测到了来自Cladophialophora,Exophiala,Rhinocladiella,Cyphellophora和Knufia属的真菌.
- 发现了克拉多菲亚洛福拉和埃克索菲亚拉的新物种.
- 首次实现了对Cladophialophora bantiana的环境隔离.
结论:
- 甘拥有多样化的黑酵母群体,包括潜在的人类病原体.
- 这项研究扩大了已知的Herpotrichiellaceae的环境息地.
- 从甘中分离Cladophialophora bantiana是一种重要的发现,因为它以前仅与人类感染有关.
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