新型遗传工具可以改善Aspergillus niger中的Penicillium expansum patulin synthase的产生
Ziyu Dai1,2
1Chemical and Biological Processes Development Group, Pacific Northwest National Laboratory, WA, Richland, USA.
The FEBS journal
|October 5, 2023
概括
研究人员开发了一种新的CRISPR-Cas9方法,以提高Aspergillus niger的蛋白质生产. 这种技术增强了特定蛋白质的表达,如工业应用的帕图林合成酶 (PatE).
科学领域:
- 分子生物学分子生物学
- 生物技术是生物技术.
- 菌类遗传学 菌类遗传学
背景情况:
- 克里斯普尔-Cas9系统已经超越了DNA裂变,用于各种生物技术用途.
- 提高工业真菌的蛋白质产量,如Aspergillus niger对于生物技术至关重要.
研究的目的:
- 开发一种新的CRISPR-Cas9介导的方法,用于增强黑色阿斯伯吉路斯菌中的转基因表达.
- 为了改善从黑色阿斯伯吉路斯中Penicillium expansum中产生的帕图林合成酶 (PatE) 的产生.
主要方法:
- 使用CRISPR-Cas9来破坏影响蛋白质产生负面影响的基因 (例如prtT,kusA).
- 葡萄糖胺酶的着陆点被整合到破坏的基因位置.
- 通过CRISPR-Cas9.9插入了PatE转基因表达盒的多个副本.
主要成果:
- 一种新的技术成功地增强了阿斯伯吉路斯尼格尔 (Aspergillus niger) 中的帕图林合成酶 (PatE) 转基因的表达.
- 该方法涉及有针对性的基因破坏和登陆地点的整合,以实现高效的转基因表达.
- 纯化PateE被用于结构和功能分析.
结论:
- 开发的CRISPR-Cas9策略有效地提高了工业状真菌中的转基因表达.
- 这种方法为增加Aspergillus niger中各种蛋白质和化学物质的生产提供了基础.
- 该技术在推进工业生物技术应用方面具有重大潜力.
关键词:
黑色阿斯珀吉勒斯 (Aspergillus niger) 是一种克里斯普尔-Cas9是什么意思KusA删除 删除 删除双链 DNA 断裂,导致双链 DNA 断裂.多拷贝集成的整合帕图林合成酶 (Patulin Synthase) 是一种蛋白质合成酶.更多相关视频
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