不同的促炎性免疫反应与牛种对结核病的不同易感性有关
Rishi Kumar1,2, Sripratyusha Gandham1,2, Avi Rana1
1National Institute of Animal Biotechnology, Hyderabad, Telangana, India.
Frontiers in immunology
|October 5, 2023
概括
土著牛对牛结核的免疫反应比杂交牛的免疫反应更强. 这种天生的免疫差异可能解释了为什么杂交牛在印度的BTB发病率更高.
科学领域:
- 兽医免疫学 兽医免疫学
- 传染性疾病 传染性疾病
- 动物传播疾病 动物传播疾病
背景情况:
- 由Mycobacterium bovis引起的牛结核病 (bTB) 是一种重要的动物传播疾病,牛作为一种储存库.
- 印度在全球结核病负担很高,但缺乏对bTB患病率的系统评估.
- 在印度,杂交牛的bTB流行率高于本土品种.
研究的目的:
- 调查导致印度本土和杂交牛群bTB发病率差异的潜在免疫反应.
- 为了比较本地 (Sahiwal) 和杂交 (Sahiwal x Holstein Friesian) 牛对bTB的易感性.
主要方法:
- 单一皮内结核素测试 (SITT) 和基于PCR的查,以检测结核菌菌复合体.
- 外周血液单核细胞 (PBMCs) 的活体感染与M. bovis BCG和M. 结核 H37Ra.
- 对细胞因子和信号通路表达的分析 (IFN-γ,IL-17,TBK-1,NO,IL-1β).
主要成果:
- 杂交的牛表现出较高的结核素反应器发生率和对M.结核综合体的PCR阳性.
- 杂交牛PBMCs显示M. bovis BCG和M. 结核 H37Ra. 的细胞内生长明显更高.
- 本地牛的PBMC诱导了更高的促炎细胞因子 (IFN-γ,IL-17,NO) 和TBK-1信号传递.
- 杂交牛PBMCs显示IL-1β转录的表达更高.
结论:
- 当地和杂交牛之间的先天免疫反应差异有助于对bTB的不同敏感性.
- 这些免疫变异可能解释了观察到的bTB发病率差异.
- 了解这些机制可以为印度的BTB控制策略提供信息.
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