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在COVID-19患者中使用元基因组下一代测序进行二次感染监测:一项跨部门研究

Renke Chen¹, Mengxiao Xie², Shenlong Wang¹

¹The First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.

Infection and drug resistance

|October 5, 2023

概括

下一代元基因组测序 (mNGS) 有效地识别了COVID-19患者的二次感染,检测了常见的病原体并预测了抗菌素耐药性. 这支持有针对性的治疗和感染控制,以改善患者的治疗结果.

科学领域:

传染性疾病传染性疾病
微生物学微生物学
基因组学就是基因组学.

背景情况:

下一代元基因组测序 (mNGS) 是诊断复杂感染和指导抗菌疗法的强大工具.
在COVID-19患者中,二次感染是一个重大问题,但使用mNGS的监测仍然未得到报道.

研究的目的:

评估支气管洗液 (BALF) mNGS在检测呼吸道病原体和预测住院COVID-19患者抗生素耐药性的实用性.
评估该患者队列中二次感染的流行率和类型.

主要方法:

mNGS对192名住院COVID-19患者的BALF样本进行分析 (2022年12月 - 2023年2月).
细菌,真菌和病毒病原体的识别.
预测抗微生物耐药性基因,并与表型耐药性数据进行比较.

主要成果:

二次感染很普遍,影响了83.3%的患者,其中细菌感染最常见 (45%).
关键的病原体包括Klebsiella pneumoniae,Acinetobacter baumannii和Candida albicans. 这两种病原体是主要的病原体.
mNGS在预测关键病原体的抗菌素耐药性方面表现出85.5%的一致性.

结论:

mNGS有效地识别二次感染,并预测COVID-19患者的抗菌素耐药性.
这种能力对于及时,有针对性的治疗和多药耐药生物的监测至关重要.

关键词:

在 COVID-19 疫情中,这就是SARS-CoV-2病毒.抗生素耐药性抗生素耐药性基因组下一代测序的测序.

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